9FVF

UMG-SP3 amidase from uncultured bacterium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.873130
Spacegroup name	P 43 21 2
Unit cell lengths	90.707, 90.707, 278.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.420 - 2.320
R-factor	0.1908
Rwork	0.188
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.141
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.420	2.408
High resolution limit [Å]	2.320	2.320
Rmerge	0.037	0.645
Rmeas	0.052
Rpim	0.037	0.645
Number of reflections	50769	4908
<I/σ(I)>	15.86	1.25
Completeness [%]	99.5	98.46
Redundancy	2
CC(1/2)	0.999	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Hepes pH 7.5 4 M Ammonium Acetate