9FTV
Serial microseconds crystallography at ID29 using MPI extruder: Thaumatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2023-04-12 |
| Detector | PSI JUNGFRAU 4M |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.490, 58.490, 151.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.280 - 1.750 |
| R-factor | 0.1422 |
| Rwork | 0.140 |
| R-free | 0.16990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.858 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.560 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 27502 | 1339 |
| <I/σ(I)> | 22.25 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2001 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | 0.1 M HEPES pH 7.5, 1.8 M Sodium Potassium Tartrate |
