9FRM

The crystal structure of glycogen phosphorylase with an indole derivative

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.7293
Spacegroup name	P 43 21 2
Unit cell lengths	125.962, 125.962, 114.924
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.530 - 2.000
R-factor	0.1795
Rwork	0.178
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.492
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.530	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.098	0.750
Number of reflections	62773	2798
<I/σ(I)>	12.2	2.7
Completeness [%]	99.9	100
Redundancy	7.6
CC(1/2)	0.998	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	6.7	289	10 mM BES