9FOE

Crystal structure of the PWWP1 domain of NSD2 bound by compound 7.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 43 21 2
Unit cell lengths	54.272, 54.272, 125.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.830 - 1.963
R-factor	0.2277
Rwork	0.227
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.830	2.104
High resolution limit [Å]	1.963	1.963
Rmerge	0.089	2.000
Rmeas	0.093	2.088
Rpim	0.027	0.594
Total number of observations		7138
Number of reflections	11750	582
<I/σ(I)>	13.4	1.5
Completeness [%]	83.2
Redundancy	11.6	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.5 M sodium formate