9FJG

Two PLK1 PBD proteins bound to CENP-U(58-114) phosphorylated at Thr98

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000696553160076
Spacegroup name	P 21 21 2
Unit cell lengths	84.670, 135.400, 60.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.050 - 2.000
R-factor	0.2128
Rwork	0.211
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.657
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.050	45.050	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.068	0.024	2.023
Rmeas	0.071	0.025	2.098
Number of reflections	47668	616	3448
<I/σ(I)>	21.32
Completeness [%]	100.0
Redundancy	13.5
CC(1/2)	1.000	1.000	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	0.02M Na3-citrate, 0.08 M NaH2(PO4) pH6.2, 18 v/v% PEG2000