9FIW

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH LRP6 INTERNALIZATION PEPTIDE SYRHFA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-05
Detector	DECTRIS PILATUS 12M
Wavelength(s)	0.928190
Spacegroup name	P 64
Unit cell lengths	127.625, 127.625, 75.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.670 - 2.820
R-factor	0.19454
Rwork	0.192
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.908
Data reduction software	xia2
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.670	2.970
High resolution limit [Å]	2.820	2.820
Rmerge	0.070	1.580
Rpim	0.022	0.500
Number of reflections	17042	2456
<I/σ(I)>	30.1
Completeness [%]	99.9
Redundancy	20.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	2.0-2.2 M NaCl 0.4M Na/K phosphate 0.1M MES PH 7.1 15-20% glycerol