9FGP
cilia and flagella associated protein 299
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-05 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 1 |
Unit cell lengths | 31.756, 34.449, 58.984 |
Unit cell angles | 91.35, 100.00, 90.09 |
Refinement procedure
Resolution | 58.071 - 1.490 |
Rwork | 0.174 |
R-free | 0.21890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.962 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.071 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Number of reflections | 28810 | 53 |
<I/σ(I)> | 1.29 | |
Completeness [%] | 71.6 | |
Redundancy | 3.5 | |
CC(1/2) | 0.998 | 0.925 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% PEG6000 -- 10% ethylene glycol -- 0.1M MES pH 6.0 -- 0.01M zinc chloride |