9F9M

Crystal structure of MUS81-EME1 bound by compound 21.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	62.902, 83.563, 91.527
Unit cell angles	90.00, 92.18, 90.00

Refinement procedure

Resolution	50.230 - 2.469
R-factor	0.307
Rwork	0.306
R-free	0.32560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.960
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.230	2.530
High resolution limit [Å]	2.470	2.470
Number of reflections	34161	2525
<I/σ(I)>	7
Completeness [%]	100.0
Redundancy	3.4
CC(1/2)	0.988	0.751

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15 % PEG10K, pH 6.5