9F9K

Crystal structure of MUS81-EME1 bound by compound 15.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	71.264, 79.066, 84.836
Unit cell angles	90.00, 91.37, 90.00

Refinement procedure

Resolution	71.240 - 2.728
R-factor	0.2338
Rwork	0.233
R-free	0.25430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.244	71.244	2.966
High resolution limit [Å]	2.728	8.334	2.728
Rmerge	0.042	0.019	0.573
Rmeas	0.058	0.026	0.808
Rpim	0.041	0.019	0.570
Number of reflections	18360	918	919
<I/σ(I)>	10.5	26.3	1.3
Completeness [%]	72.6	97.9	16.5
Redundancy	3.3	3.1	3.3
CC(1/2)	0.999	0.999	0.629

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12 % PEG8000, pH 5.5