9F98
Crystal structure of MUS81-EME1, apo form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.188, 59.377, 79.570 |
| Unit cell angles | 98.73, 105.06, 115.00 |
Refinement procedure
| Resolution | 36.660 - 2.150 |
| R-factor | 0.2182 |
| Rwork | 0.216 |
| R-free | 0.25490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.950 | 2.130 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 50038 | 2576 |
| <I/σ(I)> | 7.6 | |
| Completeness [%] | 94.9 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.996 | 0.281 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12 % PEG8K, pH 5.5 |
