9F98

Crystal structure of MUS81-EME1, apo form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	P 1
Unit cell lengths	57.188, 59.377, 79.570
Unit cell angles	98.73, 105.06, 115.00

Refinement procedure

Resolution	36.660 - 2.150
R-factor	0.2182
Rwork	0.216
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.950	2.130
High resolution limit [Å]	2.080	2.080
Number of reflections	50038	2576
<I/σ(I)>	7.6
Completeness [%]	94.9
Redundancy	3.4
CC(1/2)	0.996	0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	12 % PEG8K, pH 5.5