9F8W

Crystal structure of the apo Pex5 peroxisomal cargo receptor from Trypanosoma brucei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	H 3
Unit cell lengths	206.545, 206.545, 67.867
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.890 - 2.350
R-factor	0.20025
Rwork	0.198
R-free	0.24605
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.552
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.890	2.434
High resolution limit [Å]	2.350	2.350
Number of reflections	44917	4490
<I/σ(I)>	14.68
Completeness [%]	99.9
Redundancy	5.8	6
CC(1/2)	0.999	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.9 M sodium-potassium tartrate tetrahydrate, 20% w/v glycerol, 0.05 M Hepes pH 7.4 after 8 days at 293 K