9F7E
CtdA Canavanine tRNA-editing deacetylase from Pseudomonas canavaninivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.154, 77.902, 69.100 |
| Unit cell angles | 90.00, 92.51, 90.00 |
Refinement procedure
| Resolution | 28.280 - 1.500 |
| R-factor | 0.1591 |
| Rwork | 0.158 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS (BUILT=20230630) |
| Data scaling software | XSCALE (BUILT=20230630) |
| Phasing software | PHENIX (1.20.1_4487) |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.510 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 68888 | 1330 |
| <I/σ(I)> | 12.1 | 1.04 |
| Completeness [%] | 98.8 | 95.9 |
| Redundancy | 6.8 | 5.6 |
| CC(1/2) | 0.999 | 0.407 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289.15 | K3PO4 0.6M, Na2HPO4 1.3M, Li2SO4 0.2M, CAPS 0.1 M at pH 10.5, PEG 8K 27(v/v)%, AmSO4 0.2 M |
