9F28

Crystal structure of the heterodimeric primase from pyrococcus abyssi (deletion of the PriL-CTD domain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98011
Spacegroup name	P 43 21 2
Unit cell lengths	116.574, 116.574, 121.084
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.510 - 1.850
R-factor	0.1999
Rwork	0.199
R-free	0.22360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.510	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.114	2.080
Rpim	0.023	0.609
Number of reflections	67117	4200
<I/σ(I)>	16.4	1.6
Completeness [%]	100.0
Redundancy	26.9
CC(1/2)	0.999	0.592

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M Magnesium Formate