9F18
Crystal structure of a first-in-class antibody for alpha-1,6-fucosylated prostate-specific antigen
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-09-26 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.219, 66.735, 91.713 |
| Unit cell angles | 90.00, 112.09, 90.00 |
Refinement procedure
| Resolution | 66.010 - 2.520 |
| R-factor | 0.1871 |
| Rwork | 0.184 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.972 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.010 | 2.800 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmeas | 0.087 | 1.089 |
| Number of reflections | 19541 | 978 |
| <I/σ(I)> | 15.2 | 1.4 |
| Completeness [%] | 90.5 | 50.7 |
| Redundancy | 3.1 | 2.9 |
| CC(1/2) | 0.999 | 0.503 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 100 mM potassium thiocyanate and 30% w/v PEG MME 2000 (poly[ethylene glycol] methyl ether |
