9EY6
Crystal structure of human tyrosinase-related protein 1 (TYRP1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-24 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8731 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 103.754, 103.754, 140.988 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.967 - 2.228 |
Rwork | 0.170 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.868 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.227 | 2.240 |
Rmerge | 0.114 | 1.158 |
Rmeas | 0.130 | |
Rpim | 0.061 | 0.623 |
Number of reflections | 81266 | 3810 |
<I/σ(I)> | 6.55 | |
Completeness [%] | 99.1 | 86.53 |
Redundancy | 4.4 | 4.1 |
CC(1/2) | 0.994 | 0.460 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 6000, zinc chloride, Tris-HCl pH 8.0 |