9EY6
Crystal structure of human tyrosinase-related protein 1 (TYRP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-24 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 103.754, 103.754, 140.988 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.967 - 2.228 |
| Rwork | 0.170 |
| R-free | 0.21270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.868 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.227 | 2.240 |
| Rmerge | 0.114 | 1.158 |
| Rmeas | 0.130 | |
| Rpim | 0.061 | 0.623 |
| Number of reflections | 81266 | 3810 |
| <I/σ(I)> | 6.55 | |
| Completeness [%] | 99.1 | 86.53 |
| Redundancy | 4.4 | 4.1 |
| CC(1/2) | 0.994 | 0.460 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 6000, zinc chloride, Tris-HCl pH 8.0 |
