9EVQ
Crystal structure of the kinetoplastid kinetochore protein KKT23 acetyltransferase domain from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2020-09-22 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.266, 44.317, 68.815 |
| Unit cell angles | 100.51, 103.00, 101.32 |
Refinement procedure
| Resolution | 65.140 - 1.750 |
| R-factor | 0.1786 |
| Rwork | 0.177 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.098 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.160 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmeas | 0.083 | 0.741 |
| Number of reflections | 55472 | 3670 |
| <I/σ(I)> | 11.5 | 1.8 |
| Completeness [%] | 96.0 | 85.7 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M sodium tartrate dibasic dehydrate, 0.1 M HEPES pH 7.0, 20% SOKOLAN PA 25 CL |
