9EQJ
Crystal structure of pVHL:EloB:EloC in complex with MP-1-39
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.6702 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.237, 47.345, 99.746 |
| Unit cell angles | 81.41, 76.78, 80.49 |
Refinement procedure
| Resolution | 31.640 - 2.050 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.126 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.020 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 48840 | 1638 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 95.1 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.976 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 NaCl, 0.1M HEPES pH 7.5, and 25% PEG3350 |
