9EO5
X-ray structure of the adduct formed upon reaction of picoplatin with bovine pancreatic ribonuclease (structure C)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.750, 32.690, 73.540 |
Unit cell angles | 90.00, 90.46, 90.00 |
Refinement procedure
Resolution | 50.424 - 1.990 |
Rwork | 0.191 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.536 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.424 | 2.080 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.161 | 0.161 |
Number of reflections | 16745 | 844 |
<I/σ(I)> | 7.3 | 2.1 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 5.9 | 5.8 |
CC(1/2) | 0.988 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 22% PEG4000, 0.1 M sodium citrate pH 5.1 |