9ENJ

L-amino acid oxidase 4 (HcLAAO4) from the fungus Hebeloma cylindrosporum in complex with L-glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96770
Spacegroup name	P 1 21 1
Unit cell lengths	84.760, 130.790, 107.300
Unit cell angles	90.00, 95.66, 90.00

Refinement procedure

Resolution	40.620 - 2.100
R-factor	0.1751
Rwork	0.174
R-free	0.19500
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.002
RMSD bond angle	0.520
Data reduction software	XDS (VERSION Jun 30, 2023 BUILT=20230630)
Data scaling software	XSCALE (VERSION Jun 30, 2023 BUILT=20230630)
Phasing software	PHENIX
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmeas	0.106	1.901
Number of reflections	134327	9924
<I/σ(I)>	12.45	1.27
Completeness [%]	99.2	99.9
Redundancy	7.1	7.36
CC(1/2)	0.997	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	protein: 10 mg/ml; reservoir solution: 2.05 M (NH4)2SO4, 0.1 M sodium citrate, pH 7.0; drop with 1000 nl protein + 500 nl reservoir