9EN6

Crystal structure of RNA G2C4 repeats - native model pH 6.5

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.72932
Spacegroup name	C 2 2 21
Unit cell lengths	41.656, 45.840, 51.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.750 - 0.918
Rwork	0.118
R-free	0.12940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	2.749
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.750	0.970
High resolution limit [Å]	0.918	0.920
Number of reflections	34769	5565
<I/σ(I)>	17.2	1.59
Completeness [%]	99.9	99.8
Redundancy	13.3
CC(1/2)	0.990	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.08M Magnesium acetate tetrahydrate, 0.05M Sodium cacodylate trihydrate pH 6.5, 30% v/w Polyethylene glycol 4,000