9EN4
VP8* domain of the spike protein VP4 from bovine P[3] strain of rotavirus species C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.489, 64.370, 76.937 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.370 - 3.000 |
| R-factor | 0.24714 |
| Rwork | 0.244 |
| R-free | 0.29722 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.578 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.940 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.365 | 1.084 |
| Rmeas | 0.379 | 1.126 |
| Rpim | 0.104 | 0.303 |
| Total number of observations | 83626 | 13907 |
| Number of reflections | 6504 | 1033 |
| <I/σ(I)> | 7.7 | 3.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 12.9 | 13.5 |
| CC(1/2) | 0.986 | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | Sodium Acetate 1.5 M pH 6.5 |
