9EKO

A chimeric hybrid protein fused with the FGFR3 Transmembrane Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-06
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.920
Spacegroup name	C 1 2 1
Unit cell lengths	142.004, 70.260, 63.579
Unit cell angles	90.00, 114.18, 90.00

Refinement procedure

Resolution	64.770 - 2.901
R-factor	0.275
Rwork	0.275
R-free	0.28690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.455
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.774	3.240
High resolution limit [Å]	2.901	2.901
Rmerge	0.125	1.072
Rmeas	0.149	1.274
Rpim	0.081	0.682
Number of reflections	7012	352
<I/σ(I)>	6.3	1.5
Completeness [%]	54.8	9.9
Redundancy	3.4
CC(1/2)	0.995	0.289

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	0.04 M ammonium sulfate, 0.04 M lithium sulfate, 0.08 M Bis-Tris propane (pH 8.5) and 25 % v/v PEG Smear Broad.