9EFW

Co-crystal structure of yeast Forkhead transcription factor Fkh1 bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	P 1
Unit cell lengths	48.161, 50.245, 59.041
Unit cell angles	110.78, 100.37, 82.63

Refinement procedure

Resolution	29.510 - 2.200
R-factor	0.1914
Rwork	0.189
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21-5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.510	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.042	1.050
Rpim	0.029	0.736
Number of reflections	27067	2687
<I/σ(I)>	10.3	1.09
Completeness [%]	90.5	90.59
Redundancy	2.9	2.9
CC(1/2)	0.999	0.649

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	100 mM HEPES pH 7.0, 37.5-40% PEG 5K-MME, and 200mM of ammonium phosphate monobasic