9EFF
Crystal Structure of a nucleoid-associated protein (UBP) bound to DNA from Sulfolobus islandicus.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2022-03-03 |
Detector | RDI CMOS_8M |
Wavelength(s) | 0.91165 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.742, 136.051, 38.380 |
Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
Resolution | 36.260 - 1.820 |
R-factor | 0.2014 |
Rwork | 0.200 |
R-free | 0.22820 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.159 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.030 | 1.860 |
High resolution limit [Å] | 1.820 | 1.820 |
Rmeas | 0.115 | 1.040 |
Rpim | 0.043 | 0.416 |
Number of reflections | 31882 | 1535 |
<I/σ(I)> | 11.8 | 1.7 |
Completeness [%] | 98.4 | 96.4 |
Redundancy | 7 | 6.1 |
CC(1/2) | 0.995 | 0.884 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | Tris-HCl 0.05 M pH 8.0 - 8.5, Potassium Chloride 0.1 M, Magnesium Chloride 0.01 M and PEG400 26 - 30% |