9EFE

Crystal Structure in space group P21 of a nucleoid-associated protein (UBP) from Sulfolobus islandicus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-09-24
Detector	RDI CMOS_8M
Wavelength(s)	1.24984
Spacegroup name	P 1 21 1
Unit cell lengths	86.896, 165.538, 47.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.640 - 2.590
R-factor	0.2257
Rwork	0.222
R-free	0.25530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.640	2.600
High resolution limit [Å]	2.590	2.590
Rmeas	0.086	1.216
Rpim	0.045	0.636
Number of reflections	41929	417
<I/σ(I)>	13.2	1
Completeness [%]	99.9	100
Redundancy	3.7	3.6
CC(1/2)	0.999	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	NaAc 0.1 M pH 4.8 - 5.2, LiCl 0.2 M or MgCl2 0.2 M, and PEG6000 18 - 24%