9EFD

Crystal Structure in space group C2221 of a nucleoid-associated protein (UBP) from Sulfolobus islandicus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-09-24
Detector	RDI CMOS_8M
Wavelength(s)	1.24984
Spacegroup name	C 2 2 21
Unit cell lengths	95.010, 159.670, 47.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.430 - 2.740
R-factor	0.2679
Rwork	0.268
R-free	0.27790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.462
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.310	2.860
High resolution limit [Å]	2.730	2.730
Rmeas	0.119	1.986
Rpim	0.032	0.557
Number of reflections	9913	1279
<I/σ(I)>	22.6	1.9
Completeness [%]	99.6	99.5
Redundancy	13.8	13.2
CC(1/2)	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2 M LiCl NaAc 0.1 M pH=5 PEG6000 18-24%