Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.243, 83.243, 66.838 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.330 - 2.300 |
| R-factor | 0.2197 |
| Rwork | 0.217 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.330 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.032 | 0.559 |
| Rpim | 0.014 | 0.243 |
| Number of reflections | 12235 | 1190 |
| <I/σ(I)> | 35.5 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10 | 10 |
| CC(1/2) | 1.000 | 0.935 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M L-PROLINE, 0.1 M HEPES PH 7.5, 10% (W/V) PEG 3350, .001 M NICOTINIC ACID |
