9E7Q

Structure of a coiled-coil peptide from C. elegans EDC4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 41 3 2
Unit cell lengths	76.564, 76.564, 76.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.200 - 1.420
R-factor	0.1491
Rwork	0.148
R-free	0.16980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.025
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.200	1.440
High resolution limit [Å]	1.420	1.420
Number of reflections	15056	729
<I/σ(I)>	24.7
Completeness [%]	100.0
Redundancy	38.8
CC(1/2)	1.000	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	3M ammonium sulfate, 10% glycerol