9E70

Human XRN1 with Adenosine-3',5'-Bisphosphate (pAp) Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	60.910, 63.970, 363.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.051 - 2.100
Rwork	0.187
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.799
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.051	2.154
High resolution limit [Å]	2.100	2.100
Rmerge	0.163	1.200
Number of reflections	84285	5788
<I/σ(I)>	10.9	2.1
Completeness [%]	100.0	100
Redundancy	13.1	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	0.2 M Potassium fluoride, pH 7.3, 20% w/v PEG 3350