9E6S

BCL11A ZF4-6 with K784T Mutation in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGACCA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 41 21 2
Unit cell lengths	59.464, 59.464, 246.988
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.850 - 2.200
R-factor	0.2423
Rwork	0.241
R-free	0.27470
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.005
RMSD bond angle	0.636
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.850	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.136
Rpim	0.038
Number of reflections	39233	2021
<I/σ(I)>	8.8	0.3
Completeness [%]	99.8	99.7
Redundancy	14.1	13.7
CC(1/2)	0.998	0.484

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	292	18 % w/v PEG 3350, 17 % v/v PEG 400, 4.8 % v/v 2-Propanol, 0.1 M CAPSO 9.0