Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.920, 101.500, 66.800 |
| Unit cell angles | 90.00, 90.64, 90.00 |
Refinement procedure
| Resolution | 43.330 - 1.080 |
| R-factor | 0.1525 |
| Rwork | 0.151 |
| R-free | 0.17780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.330 | 1.190 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmerge | 0.980 | |
| Number of reflections | 255378 | 19081 |
| <I/σ(I)> | 8.23 | |
| Completeness [%] | 93.8 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.998 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MES 6 pH, 0.1-0.2 M NaCl, 21-26 %w/v PEG 3350 |
