9DZN
KAT6A MYST domain complexed with a H3K14-CoA bisubstrate inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2022-06-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.368, 59.368, 210.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.130 - 1.724 |
| R-factor | 0.22 |
| Rwork | 0.219 |
| R-free | 0.24470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (3-FEB-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.368 | 1.786 |
| High resolution limit [Å] | 1.724 | 1.724 |
| Number of reflections | 503914 | 29171 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 91.8 | 56.2 |
| Redundancy | 13 | 10.6 |
| CC(1/2) | 0.998 | 0.753 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 294.15 | 1.25 M NH4SO4, 0.28 M NaCl, and 0.1 M HEPES pH 6.4 |
