9DZM
Dimeric human OCT2 (POU2F2) POU domain bound to palindromic MORE DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-07-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.029, 54.922, 69.164 |
| Unit cell angles | 82.37, 79.66, 71.77 |
Refinement procedure
| Resolution | 33.910 - 2.540 |
| R-factor | 0.2197 |
| Rwork | 0.217 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.910 | 2.631 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.062 | 0.402 |
| Rmeas | 0.084 | 0.544 |
| Rpim | 0.056 | 0.364 |
| Number of reflections | 16395 | 1603 |
| <I/σ(I)> | 8.37 | 2.03 |
| Completeness [%] | 95.5 | 95.19 |
| Redundancy | 2.1 | 2 |
| CC(1/2) | 0.995 | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 295 | 150 mM KBr, 27.5% PEG MME 2000 mixed 1:1 with 200 uM DNA:protein complex |
