9DXX

Crystal structure of the A/Puerto Rico/8/1934 (H1N1) influenza virus hemagglutinin in complex with D-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	193
Detector technology	PIXEL
Collection date	2015-02-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97962
Spacegroup name	H 3 2
Unit cell lengths	107.693, 107.693, 387.162
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.020 - 2.370
R-factor	0.197
Rwork	0.195
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.932
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.370	2.370
Number of reflections	35478	35482
<I/σ(I)>	23.7
Completeness [%]	100.0
Redundancy	10.4
CC(1/2)	0.970	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.2M Tri-potassium citrate, 20% w/v PEG3350