9DU0
Crystal structure of the bromodomain of human BRM (SMARCA2) in complex with a SMARCA-BD binder
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 31 |
| Unit cell lengths | 64.558, 64.558, 89.083 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.400 - 3.000 |
| R-factor | 0.2285 |
| Rwork | 0.223 |
| R-free | 0.33810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.132 | 0.827 |
| Rmeas | 0.170 | 1.073 |
| Rpim | 0.105 | 0.674 |
| Number of reflections | 8198 | 1331 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 98.4 | |
| Redundancy | 2.3 | |
| CC(1/2) | 0.992 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.025~0.1 M zinc acetate, 16~26% w/v PEG3350 |
