9DR9

Binary product complex of DNA polymerase iota with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-09-11
Detector	NOIR-1
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	97.100, 97.100, 202.044
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.760 - 2.210
R-factor	0.257
Rwork	0.255
R-free	0.29720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.834
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	2.290
High resolution limit [Å]	2.060	2.210
Rpim	0.018	0.419
Number of reflections	28209	2057
<I/σ(I)>	25.8	1.7
Completeness [%]	100.0	100
Redundancy	18.9	11.6
CC(1/2)	1.000	0.766

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	100 mM HEPES pH 7 1.5 - 1.8 M Ammonium Sulfate 5 mM EDTA