9DPY
BMP-10 with BMP-9 Crystal Contacts
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-11-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.752, 123.038, 120.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.520 - 1.770 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.962 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.520 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.078 | 2.426 |
| Rmeas | 0.088 | 2.727 |
| Rpim | 0.040 | 1.237 |
| Number of reflections | 31025 | 4391 |
| <I/σ(I)> | 12.2 | 1 |
| Completeness [%] | 98.8 | 97.2 |
| Redundancy | 8.9 | 8.9 |
| CC(1/2) | 0.998 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 25% (v/v) 1,2-propanediol, 100 mM sodium phosphate dibasic/potassium phosphate monobasic pH 6.2, 10% (v/v) glycerol |
