9DPS
BMP-9 G389S Dimer Without Radiation Damage in Neutral pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 70.774, 70.774, 145.525 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.820 - 2.060 |
| R-factor | 0.2179 |
| Rwork | 0.216 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.038 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.650 | 2.120 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.111 | 1.004 |
| Rmeas | 0.130 | 1.177 |
| Rpim | 0.066 | 0.596 |
| Number of reflections | 11744 | 903 |
| <I/σ(I)> | 7.2 | 2.1 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 5.8 | 6 |
| CC(1/2) | 0.993 | 0.794 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 1M NaCl, 22-26% glycerol, 0.1 M HEPES pH 7.5 |
