9DOC

The von Willebrand factor A domain of human capillary morphogenesis gene II, flexibly fused to the 1TEL crystallization chaperone, Thr-Val linker variant, at 1.2 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 65
Unit cell lengths	101.428, 101.428, 49.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.920 - 1.190
R-factor	0.1894
Rwork	0.189
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.929
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.920	1.233
High resolution limit [Å]	1.190	1.190
Rmerge	0.047	2.286
Rmeas	0.050	2.463
Rpim	0.016	0.895
Number of reflections	91358	7752
<I/σ(I)>	15.17	0.62
Completeness [%]	97.2
Redundancy	9
CC(1/2)	1.000	0.523

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	298	100 mM Bis-Tris, pH 7.25, 1350 mM Sodium Potassium Phosphate