9DL0
Crystal structure of a synthetic Fab (R3H8) in complex with the FRB domain of mTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 87.613, 110.042, 131.079 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.310 - 2.000 |
| R-factor | 0.2065 |
| Rwork | 0.205 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.014 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.1)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 110.200 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 82556 | 8485 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 95.8 | |
| Redundancy | 2 | |
| CC(1/2) | 0.998 | 0.295 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M HEPES pH 7.5 4% PEG 400 2.2 M ammonium sulfate |
