9DIQ

Crystal structure of Apo-H5 hemagglutinin from the influenza virus A/Texas/37/2024 (H5N1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	130.367, 72.061, 206.859
Unit cell angles	90.00, 91.66, 90.00

Refinement procedure

Resolution	33.740 - 2.690
R-factor	0.2125
Rwork	0.210
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.740	2.750
High resolution limit [Å]	2.690	2.690
Rmeas	0.220	1.700
Rpim	0.110	0.840
Number of reflections	103266	9020
<I/σ(I)>	8.5
Completeness [%]	96.1
Redundancy	3.9
CC(1/2)	0.930	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Hepes 16% PEG6000