9DIP

Crystal structure of H5 hemagglutinin from the influenza virus A/Texas/37/2024 (H5N1) with LSTa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	103.539, 74.760, 131.101
Unit cell angles	90.00, 96.21, 90.00

Refinement procedure

Resolution	31.180 - 2.320
R-factor	0.2185
Rwork	0.216
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21rc1_5127: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.380	2.360
High resolution limit [Å]	2.320	2.320
Rmeas	0.150	2.000
Rpim	0.060	0.780
Number of reflections	85509	8523
<I/σ(I)>	15.7
Completeness [%]	99.0
Redundancy	6.7
CC(1/2)	0.980	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1M Hepes 20% PEG6000