9DBO

Crystal structure of a synthetic Fab (R3E9) in complex with the FRB domain of mTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 2 1
Unit cell lengths	45.055, 69.732, 89.413
Unit cell angles	90.00, 90.12, 90.00

Refinement procedure

Resolution	45.100 - 1.550
R-factor	0.18521
Rwork	0.184
R-free	0.21696
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (v1.21.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	75458	5684
<I/σ(I)>	6.7
Completeness [%]	94.0
Redundancy	1.9
CC(1/2)	0.994	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M Calcium chloride dihydrate 16% PEG 3350