9D9R
X-ray structure of ALX4 homeodomain dimer bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 277 |
| Detector technology | PIXEL |
| Collection date | 2022-12-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 70.460, 70.460, 159.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.210 - 2.389 |
| R-factor | 0.2149 |
| Rwork | 0.214 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.820 |
| Data reduction software | MOSFLM |
| Data scaling software | pointless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.4 (23-JAN-2024)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.570 | 2.480 |
| High resolution limit [Å] | 2.389 | 2.390 |
| Rmerge | 0.150 | 3.460 |
| Rmeas | 0.158 | 3.657 |
| Rpim | 0.050 | 1.169 |
| Total number of observations | 18662 | |
| Number of reflections | 18846 | 1933 |
| <I/σ(I)> | 4.7 | 0.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 10.7 | 9.7 |
| CC(1/2) | 0.987 | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1M Tris pH 7.5, 0.2M Trimethylamine N-oxide, 20% PEG MME 2000 |
