9D1Z

Structure of G75Q Ubiquitin bound to KLHDC3-EloB/C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97741
Spacegroup name	C 2 2 21
Unit cell lengths	114.949, 120.873, 152.949
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.950 - 1.880
R-factor	0.1697
Rwork	0.169
R-free	0.19030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.975
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.970
High resolution limit [Å]	1.880	4.090	1.900
Rmerge	0.092	0.048	0.777
Rmeas	0.099	0.051	0.838
Rpim	0.035	0.018	0.310
Number of reflections	85054	8834	8378
<I/σ(I)>	5.9
Completeness [%]	100.0	100	100
Redundancy	8.1	8.1	7.4
CC(1/2)	0.997	0.998	0.789

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	10%%KMME, 5% Tacsimate pH=7.0, 0.1M HEPES 7.0