9D1X
Crystal structure of FGFR3 bound to indazole inhibitor
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-27 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 55.870, 55.870, 365.784 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.230 - 1.600 |
| Rwork | 0.180 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.250 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.123 | 1.084 |
| Rmeas | 0.125 | 1.103 |
| Rpim | 0.022 | 0.199 |
| Total number of observations | 1444088 | |
| Number of reflections | 46623 | 2209 |
| <I/σ(I)> | 15.6 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 31 | 28.1 |
| CC(1/2) | 0.999 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 293 | 0.2 M magnesium chloride, 0.3 M sodium chloride, 23% (w/v) PEG 3350, 0.1 M Tris-HCl pH 8.1 |
