Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 67.117, 71.454, 176.452 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 1.880 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.827 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.062 | 1.479 |
| Rmeas | 0.068 | 1.602 |
| Rpim | 0.026 | 0.610 |
| Total number of observations | 467837 | 28002 |
| Number of reflections | 69452 | 4240 |
| <I/σ(I)> | 12.1 | 1.1 |
| Completeness [%] | 99.6 | |
| Redundancy | 6.7 | 6.6 |
| CC(1/2) | 1.000 | 0.642 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.97 | 281 | 0.2 M Lithium Acetate 10% w/v PEG 8000 0.1 M trisodium citrate-citric acid pH 5.97 0.44 mM Inhibitor |
