9CXL

Crystal structure of IgG1 FC WT at pH 5.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	110
Detector technology	PIXEL
Collection date	2024-01-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.972
Spacegroup name	C 1 2 1
Unit cell lengths	67.385, 111.454, 63.660
Unit cell angles	90.00, 95.74, 90.00

Refinement procedure

Resolution	32.500 - 2.330
R-factor	0.2307
Rwork	0.225
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.106
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.500	2.450
High resolution limit [Å]	2.330	2.330
Rmerge	0.194	1.700
Rmeas	0.210	0.950
Rpim	0.078	0.900
Total number of observations	141675
Number of reflections	19890	2876
<I/σ(I)>	5.4
Completeness [%]	99.4
Redundancy	7.1	7
CC(1/2)	0.995	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	295	1 M Ammonium sulfate, 0.1 M Sodium citrate pH 5.5