Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.830, 101.280, 65.710 |
| Unit cell angles | 90.00, 91.27, 90.00 |
Refinement procedure
| Resolution | 65.690 - 1.350 |
| R-factor | 0.1508 |
| Rwork | 0.149 |
| R-free | 0.18550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.826 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.690 | 1.398 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.794 | |
| Number of reflections | 132313 | 12578 |
| <I/σ(I)> | 5.61 | |
| Completeness [%] | 96.7 | |
| Redundancy | 3.9 | |
| CC(1/2) | 0.990 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 3350 + 0.20M Sodium chloride + 0.10M MES pH 5.5 + 1.25% Glycerol |
