9CSL
Structure of mutant human geranylgeranyl pyrophosphate synthase (Y246D-C247L) in complex with isopentenyl pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 191.110, 191.110, 96.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.555 - 2.100 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.21590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.560 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.101 | 1.683 |
| Rmeas | 0.104 | 1.736 |
| Rpim | 0.023 | 0.419 |
| Number of reflections | 60724 | 2941 |
| <I/σ(I)> | 20.4 | 1.6 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 19.6 | 16.9 |
| CC(1/2) | 1.000 | 0.665 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 0.2 M di-ammonium phosphate, 2.2 M ammonium sulfate |
